Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm5a/seq/ZK524.2b
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Query: ZK524.2b CE15287 locus:unc-13 UNC-13 protein status:Partially_confirmed SW:P27715
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
C2 C2 domain 167.1 9.1e-47 2
DAG_PE-bind Phorbol esters/diacylglycerol binding d 86.8 4.5e-22 1
VPS9 Vacuolar sorting protein 9 (VPS9) domai 8.2 0.95 1
GerE Bacterial regulatory proteins, luxR fam 6.3 4 1
CARD Caspase recruitment domain 4.5 8.3 1
Prothymosin Prothymosin/parathymosin family 4.1 4.1 1
LexA_DNA_bind LexA DNA binding domain 3.8 5.5 1
DUF615 Protein of unknown function (DUF615) 2.9 6.2 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
DAG_PE-bind 1/1 173 222 .. 1 51 [] 86.8 4.5e-22
DUF615 1/1 235 271 .. 142 182 .] 2.9 6.2
Prothymosin 1/1 363 381 .. 94 111 .] 4.1 4.1
C2 1/2 297 388 .. 1 88 [] 110.4 2.1e-30
CARD 1/1 469 485 .. 74 90 .] 4.5 8.3
VPS9 1/1 790 805 .. 103 118 .] 8.2 0.95
GerE 1/1 1031 1042 .. 1 12 [. 6.3 4
LexA_DNA_bind 1/1 1031 1043 .. 1 13 [. 3.8 5.5
C2 2/2 1128 1218 .. 1 88 [] 59.3 1.2e-15
Alignments of top-scoring domains:
DAG_PE-bind: domain 1 of 1, from 173 to 222: score 86.8, E = 4.5e-22
*->HrFkrttfyksptfCdhCgellwglakQGlkCsnCglnvHkrChekV
H+F ttf ++ptfC C+ llwgla+QGl+C++C+++vH++C e++
ZK524.2b 173 HNFATTTF-QTPTFCYECEGLLWGLARQGLRCTQCQVKVHDKCRELL 218
ptnC<-*
++C
ZK524.2b 219 SADC 222
DUF615: domain 1 of 1, from 235 to 271: score 2.9, E = 6.2
*->eADR.QkLRtLiRnAkKEkaqNKPPkafReiFQyLkelmetq<-*
eADR+Q L + iR K ++qNKP e+FQ+++++ +
ZK524.2b 235 EADRtQSLVNVIRDRMKIQEQNKP-----EVFQMIRTVFDVD 271
Prothymosin: domain 1 of 1, from 363 to 381: score 4.1, E = 4.1
*->DEDdDvetK.KqKktDEDD<-*
DED+D K +qK t E D
ZK524.2b 363 DEDNDLKSKlRQKLTRESD 381
C2: domain 1 of 2, from 297 to 388: score 110.4, E = 2.1e-30
*->LrVtvieAknLppkDkkngksDPYVkvslggdpkdqkkkTkvikktl
+++tv++A++L +kD k+gksDPYV+ ++g+ +k++T++i+ l
ZK524.2b 297 ITLTVLCAQGLIAKD-KTGKSDPYVTAQVGK----TKRRTRTIHQEL 338
NPvWNEtFtFevpppdlqetLeitVyDydrfgr...........ddflGe
NPvWNE+F+Fe ++++ ++ +++ V+D+d +++ +++ ++++ddflG+
ZK524.2b 339 NPVWNEKFHFE-CHNSTDR-IKVRVWDEDNDLKsklrqkltresDDFLGQ 386
vs<-*
++
ZK524.2b 387 TV 388
CARD: domain 1 of 1, from 469 to 485: score 4.5, E = 8.3
*->aLretdqelladlllde<-*
+++et+qe ++++++++
ZK524.2b 469 CFQETGQEIAEEFAMRY 485
VPS9: domain 1 of 1, from 790 to 805: score 8.2, E = 0.95
*->LTsLeaAleFiktlde<-*
+T+L aAl+++k +d+
ZK524.2b 790 FTQLNAALKLLKQMDC 805
GerE: domain 1 of 1, from 1031 to 1042: score 6.3, E = 4
*->ldsLspREreVL<-*
+sL+pR+++VL
ZK524.2b 1031 EKSLTPRQCTVL 1042
LexA_DNA_bind: domain 1 of 1, from 1031 to 1043: score 3.8, E = 5.5
*->MkpLTeRQrevLd<-*
k LT+RQ +vLd
ZK524.2b 1031 EKSLTPRQCTVLD 1043
C2: domain 2 of 2, from 1128 to 1218: score 59.3, E = 1.2e-15
*->LrVtvieAknLppkDkkngksDPYVkvslggdpkd.qk..kkTkvik
++V+++ A +L+ + ++ P+V+v+l g + ++k++ Tk++
ZK524.2b 1128 VTVKILAANDLRW-Q-TSSAFKPFVEVHLVGPHLSdKKrkWSTKTKA 1172
ktlNPvWNEtFtFe.vpppdlqe.tLeitVyDydrfgrddflGevs<-*
+++ P++NEtF+F ++ + ++ +L ++V+Dy+ + d ++G +
ZK524.2b 1173 GNWAPKFNETFHFFlGNEGEPEHyELMFQVKDYCFARDDRVVGVGV 1218