Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm4/seq/T25C12.3
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Query: T25C12.3 CE18966 EGF-repeats status:Partially_confirmed TR:Q22774 protein_id:CAA91487.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
vwa von Willebrand factor type A domain 28.8 3.9e-08 1
lectin_c Lectin C-type domain 19.9 0.001 1
EGF EGF-like domain 6.3 4.6 6
DSL Delta serrate ligand 6.2 1.5 3
metalthio Metallothionein 5.4 5.2 1
Lipase_3 Lipase (class 3) 4.8 2.1 1
DUF427 Domain of unknown function (DUF427) 3.9 9.1 1
urease_gamma Urease, gamma subunit 3.6 4.1 1
Arthro_defensin Arthropod defensin 3.0 5.2 1
gpdh Glyceraldehyde 3-phosphate dehydrogen -0.1 8.6 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
EGF 1/6 221 248 .. 7 45 .] 8.2 1.3
DSL 1/3 268 277 .. 58 67 .] 0.3 96
EGF 2/6 266 277 .. 29 45 .] 8.3 1.2
metalthio 1/1 259 278 .. 1 26 [. 5.4 5.2
Lipase_3 1/1 293 355 .. 1 70 [. 4.8 2.1
DSL 2/3 718 728 .. 57 67 .] 7.1 0.85
EGF 3/6 699 728 .. 2 45 .] 9.3 0.62
EGF 4/6 732 755 .. 12 45 .] 8.0 1.4
urease_gamma 1/1 1000 1009 .. 83 92 .. 3.6 4.1
gpdh 1/1 1012 1023 .. 28 39 .. -0.1 8.6
lectin_c 1/1 1052 1124 .. 1 82 [. 19.9 0.001
Arthro_defensin 1/1 1332 1338 .. 30 36 .] 3.0 5.2
EGF 5/6 1317 1346 .. 1 45 [] 5.5 7.7
DSL 3/3 1332 1346 .. 53 67 .] 6.9 0.97
EGF 6/6 1351 1375 .. 13 45 .] 4.0 21
DUF427 1/1 1623 1635 .. 93 105 .] 3.9 9.1
vwa 1/1 1825 1994 .. 1 189 [] 28.8 3.9e-08
Alignments of top-scoring domains:
EGF: domain 1 of 6, from 221 to 248: score 8.2, E = 1.3
*->CsngGtCvntpggssdnfggytCeCppGdyylsytGkrC<-*
C n+Gt +n+ + +g tC+C + ++Gk+C
T25C12.3 221 CFNNGTLLNVAN------QGQTCFCSEL-----FNGKQC 248
DSL: domain 1 of 3, from 268 to 277: score 0.3, E = 96
*->CleGWkGeyC<-*
C++G G +C
T25C12.3 268 CAAGYHGTNC 277
EGF: domain 2 of 6, from 266 to 277: score 8.3, E = 1.2
*->CeCppGdyylsytGkrC<-*
CeC+ G y+G +C
T25C12.3 266 CECAAG-----YHGTNC 277
metalthio: domain 1 of 1, from 259 to 278: score 5.4, E = 5.2
*->mDPqdCnCaeggsCsCgtsCkCtnCk<-*
+DP g+ C+C+++ +tnC+
T25C12.3 259 PDP------DGNLCECAAGYHGTNCQ 278
Lipase_3: domain 1 of 1, from 293 to 355: score 4.8, E = 2.1
*->rrdIvvafRGTltllellddlliflttdvtpfefdgninveddqddp
++++vv++R T++++++l+ + + + + d +
T25C12.3 293 YKTLVVIIRSTVSMNQNLNAISSA---IYKELTSN---------DGN 327
vvkvhggfvdlyfhrg.....flslrdq<-*
+ v +gfv +f +g +++++ ++dq
T25C12.3 328 NYEVYKGFVIVKFANGaytntYYPAFDQ 355
DSL: domain 2 of 3, from 718 to 728: score 7.1, E = 0.85
*->lCleGWkGeyC<-*
+C++G +G+yC
T25C12.3 718 ICPPGFYGQYC 728
EGF: domain 3 of 6, from 699 to 728: score 9.3, E = 0.62
*->apnnpCsngGtCvntpggssdnfggytCeCppGdyylsytGkrC<-*
+pn C+ngG+ ++ + tC+CppG + G +C
T25C12.3 699 PPN-SCQNGGV-TDPTN-------NATCICPPG-----FYGQYC 728
EGF: domain 4 of 6, from 732 to 755: score 8.0, E = 1.4
*->tCvntpggssdnfggytCeCppGdyylsytGkrC<-*
+Cvn++ g C+Cp+G +G +C
T25C12.3 732 QCVNGGT-----PRGGMCVCPVG-----TAGTFC 755
urease_gamma: domain 1 of 1, from 1000 to 1009: score 3.6, E = 4.1
*->VEAtFPDGTK<-*
VE+tFPDG K
T25C12.3 1000 VEVTFPDGQK 1009
gpdh: domain 1 of 1, from 1012 to 1023: score -0.1, E = 8.6
*->rsdieVVAINDl<-*
+sd e+VAIND+
T25C12.3 1012 NSDYELVAINDP 1023
[output cut off at A = 10 top alignments]