Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm3/seq/F41C3.3
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Query: F41C3.3 CE31935 Long-chain-fatty-acid coA ligase status:Partially_confirmed TR:Q20264 protein_id:AAC46810.2
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
AMP-binding AMP-binding enzyme 353.4 2.5e-102 1
DUF251 Putative 5'-3' exonuclease domain 3.9 1.2 1
K_tetra K+ channel tetramerisation domain 3.7 8.8 1
Glyco_hydro_63 Mannosyl oligosaccharide glucosidase 1.0 9.1 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
K_tetra 1/1 20 37 .. 94 111 .] 3.7 8.8
Glyco_hydro_63 1/1 26 55 .. 1 38 [. 1.0 9.1
DUF251 1/1 301 314 .. 1 13 [. 3.9 1.2
AMP-binding 1/1 92 472 .. 1 373 [] 353.4 2.5e-102
Alignments of top-scoring domains:
K_tetra: domain 1 of 1, from 20 to 37: score 3.7, E = 8.8
*->eFygldelaiesCcedeY<-*
+Fy +++ +++Cc+ ++
F41C3.3 20 KFYLISKISLSLCCIIRF 37
Glyco_hydro_63: domain 1 of 1, from 26 to 55: score 1.0, E = 9.1
*->vllsmlGmikraivsnrlvrhfwlldgyleprvntvfp<-*
+ ++ l +i r hf+l+++ l+++v+ v+p
F41C3.3 26 KISLSLCCIIR--------FHFLLFLEMLSRLVSSVHP 55
DUF251: domain 1 of 1, from 301 to 314: score 3.9, E = 1.2
*->MGVPaFF.RWLser<-*
MGVP FF+R L+ +
F41C3.3 301 MGVPTFFsRLLASK 314
AMP-binding: domain 1 of 1, from 92 to 472: score 353.4, E = 2.5e-102
*->TYrELderanrlAaaLr.alGvgpGdrVaillpnspelvvailAvlk
TY+E+ r + A aL +++ +++GdrV+ + ++ ++ +++ A+l
F41C3.3 92 TYGEFVKRTGQYATALTeKYNIKKGDRVMARVSKTTDTAALYIACLQ 138
aGaayvPldpsypaerlayiledsgakllltddallarl...........
+Ga y P++p+y++ + a+ ++d+ +++l+ + + l+++ ++ + ++
F41C3.3 139 IGALYIPVNPGYTESEAAHYIKDATPSILVSCNEELDKVfrdkirvined 188
.........s.e.p.ppvdpddlAyiiYTSGTTGkPKGVmltHrnlvnlv
+ ++++++ + + v d+A ++YTSGTTG+PKG++ltH++l n++
F41C3.3 189 klaseagslNaCtMiEHVEKSDPASVCYTSGTTGLPKGAILTHGSLSNNA 238
aala.rf.l.pddaervlllapplyhfdgsvwelfgpLlaGatlvlvpkf
+++ + ++++ +d l+ +p +yh++g+ ++l ++L++ t++ +kf
F41C3.3 239 HDIVrDWgFtGND--YNLHALP-FYHVHGLYYSLHCSLFSHSTMIWRSKF 285
tryrdp.alldliekykvTvlflvPtllrlllaape....dlssLrlvls
+++ ++++ ++Tv+++vPt++ +lla+ + +++ + ++r+++s
F41C3.3 286 E----VeDCIKYMK--NATVMMGVPTFFSRLLASKNfnkeAFGNVRVFIS 329
gGealppellrrlrerfgsggvrlvngYGpTEttvvatanppg.d.pgsi
g ++l++++++++rer+g +++ YG+TE++v++t + g+ ++g++
F41C3.3 330 GSAPLSVSTIEEFRERTG---QVILERYGMTEAGVMTTNPLNGeRkAGTV 376
GrPlpgvevyvlDeegrpvppGevGELyirGwpgvarGYlnrPelTaerF
G+++ gv ++ + G + ++ ++ GY+++P +Tae F
F41C3.3 377 GPAVQGVGCR----------IAKNGGIEVKT-NAIFAGYWKNPKKTAEEF 415
vadgwgRmYrTGDlarwdpDGyleilGRiDdqvKirGerIepgEiEavLl
++dgw ++TGD++ +d+DGyl+i GR++d+v+ +G +++p E+E+ +
F41C3.3 416 TEDGW---FKTGDVGHLDEDGYLTIGGRSKDMVITGGLNVYPKELEDFID 462
ehPgVaeAaV<-*
++P V+e+aV
F41C3.3 463 TLPFVKESAV 472