Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm2/seq/F30F8.2
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Query: F30F8.2 CE28553 glutaminase status:Partially_confirmed SW:Q93650 protein_id:CAB03030.2
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
Glutaminase Glutaminase 640.8 7.4e-189 1
ank Ankyrin repeat 22.7 0.00012 3
LAG1 Longevity-assurance protein (LAG1) 5.2 2.3 1
OKR_DC_1_N Orn/Lys/Arg decarboxylase, N-terminal dom 3.8 3.8 1
GRASP55_65 GRASP55/65 family 3.3 7.9 1
DUF111 Protein of unknown function DUF111 2.6 4 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
LAG1 1/1 156 177 .. 216 237 .] 5.2 2.3
OKR_DC_1_N 1/1 300 319 .. 118 139 .] 3.8 3.8
DUF111 1/1 406 420 .. 1 16 [. 2.6 4
Glutaminase 1/1 174 460 .. 1 290 [] 640.8 7.4e-189
ank 1/3 482 514 .. 1 33 [] 9.1 0.88
ank 2/3 515 548 .. 1 33 [] 10.6 0.32
ank 3/3 549 569 .. 1 21 [. 3.0 46
GRASP55_65 1/1 573 581 .. 1 9 [. 3.3 7.9
Alignments of top-scoring domains:
LAG1: domain 1 of 1, from 156 to 177: score 5.2, E = 2.3
*->rvltdrGelteDvRSDsEsdde<-*
r+++d Ge+ eDvRS+ E+d +
F30F8.2 156 RFVSDMGEIFEDVRSHNEGDLA 177
OKR_DC_1_N: domain 1 of 1, from 300 to 319: score 3.8, E = 3.8
*->yalegdaedyarqietAarnrY<-*
y++ +d+ ++ +++etA r +Y
F30F8.2 300 YIG-FDNSVFLSERETADR-NY 319
DUF111: domain 1 of 1, from 406 to 420: score 2.6, E = 4
*->vaifDafaGiSGDMiL<-*
+ ++a +G+SGDMi+
F30F8.2 406 -VGLPAKSGVSGDMII 420
Glutaminase: domain 1 of 1, from 174 to 460: score 640.8, E = 7.4e-189
*->GkvAdYIPaLAkvdpdllGiavvtvDGqvysaGDadepFslQSISKV
G++A+YIP+L++v pd+++++v+t+DGq+ ++GDa +pF+lQS+SK+
F30F8.2 174 GDLATYIPQLSRVAPDSWAMSVCTIDGQRKMWGDALKPFCLQSVSKP 220
ftlalaledlGeeyvwsrVGkEPSGdpFNSlvqLEtekpGkPrNPmINAG
ft+al+++d+G+e+++ +VG+EPSG+ FN++++++++k P+NP+INAG
F30F8.2 221 FTYALVHDDIGPEELHAHVGQEPSGRLFNDISLDHNKK---PHNPLINAG 267
AIvvtsllkgrttiaerldflLsflrklaGneeigideeVaqSEreTadR
AIvv+sllk+++++a+r+df+++ +rk++G+ +ig+d+ V++SEreTadR
F30F8.2 268 AIVVASLLKNKLPLADRFDFMIHACRKFVGSGYIGFDNSVFLSERETADR 317
NrAlAyymkskGnfekdveLkavLdvYfqqCalemsCedLAvigatLAng
N+Al+yym+++++f+kd +L+++Ld+Yfq+C++e++C++LAv++atLAng
F30F8.2 318 NYALSYYMREHKVFPKDLNLQDTLDLYFQICSIETNCDSLAVMAATLANG 367
GvnPitgerVvspdvarrilAlMlTCGmYdaSGdFAyrVGLPAKSGVsGG
GvnP++ger+v+++++r++l+lM++CGmYd+SG+FA++VGLPAKSGVsG+
F30F8.2 368 GVNPMNGERIVNNRACRDTLSLMYSCGMYDWSGQFAFHVGLPAKSGVSGD 417
IlaVVPgvmGiavwSPpLDelGNSvaGvaaLekLaqrtglsiF<-*
+++V+P+vmGia++SP+LD+lGN+v+Gv+++e+L+q+++++++
F30F8.2 418 MIIVIPNVMGIALYSPRLDKLGNTVRGVKFAEQLVQKYNFHNY 460
ank: domain 1 of 3, from 482 to 514: score 9.1, E = 0.88
*->dGnTPLHlAakrgnlevvklLlqrGAdlnaqdd<-*
dG+ ++A k g++ +k++l G d++ +d
F30F8.2 482 DGQNRFMYATKLGDIAAIKRFLLMGHDIHCKDY 514
ank: domain 2 of 3, from 515 to 548: score 10.6, E = 0.32
*->dGnTPLHlAakrgnlevvklLlq.rGAdlnaqdd<-*
d +T LH+Aa g++ +++ l++ d+n d+
F30F8.2 515 DDRTVLHVAAAEGDVVTLEYVLSkWQEDPNPCDR 548
ank: domain 3 of 3, from 549 to 569: score 3.0, E = 46
*->dGnTPLHlAakrgnlevvklL<-*
+ +TPL A + +++ +vklL
F30F8.2 549 YDRTPLDDAKHFNHTACVKLL 569
GRASP55_65: domain 1 of 1, from 573 to 581: score 3.3, E = 7.9
*->LeVyssKTq<-*
++Vy++K+q
F30F8.2 573 ITVYNLKGQ 581